Human Dental Pulp Stem Cell Osteogenic Differentiation Seeded on Equine Bone Block with Graphene and Melatonin

Equine bone blocks have osteogenic effects promoting regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization such scaffolds. Melatonin is able to improve health mediate formation. Collagenated equine were coated ammonia-functionalized graphene-oxide (G-N) at two different concentrations (2 μg/mL, G-N2; 10 G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us quantify the amount deposited on blocks. aim this study investigate in vitro effect G-N-coated collagenated proliferation differentiation hDPSCs addition a melatonin. This evaluation determined after 7, 14, 21 days culture expression specific microRNAs, RUNX2 SMAD5 gene expression, osteocalcin levels, histological analysis. results showed that G-N2 G-N10 melatonin gave an optimal cell adhesion as shown analysis, increase potential confirmed microRNA levels. suggests promote process.